Exploring the Mechanism of MicroRNA-125a Reversing the Resistance of Laryngeal Cancer Stem Cells to Cisplatin from Mitochondrial Autophagy

To explore the mechanism of microRNA-125a reversing resistance laryngeal cancer stem cells to cisplatin from mitochondrial autophagy. The human carcinoma cell line Hep-2 was purchased Shanghai Bioleaf Biotechnology Co., Ltd. expression messenger RNA in each group analyzed by reverse transcription-polymerase chain reaction. migration and invasion ability evaluated Transwell assay. Cell apoptosis detected flow cytometry. Western blot analysis conducted evaluate protein expression. Hep-2/diamminedichloroplatinum lower than that control (p<0.05) mimic higher (p<0.05). Compared with group, had more fragments average branch length mitochondria decreased migration, viability increased rate phosphoinositide 3-kinase, kinase B mammalian target rapamycin their Hep-2/ diamminedichloroplatinum dysregulation related induced through 3-kinase/protein B/mammalian pathway mediated autophagy inhibition. Overexpression inhibited growth metastasis 3-kinase/